Microdot (CPython and MicroPython) Python

import binascii  # available on MicroPython as well as CPython

from microdot import Microdot

GIF = binascii.a2b_base64("R0lGODlhAQABAIAAAP///wAAACH5BAEAAAAALAAAAAABAAEAAAICRAEAOw==")

app = Microdot()


@app.get("/pixel.gif")
async def pixel(request):
    return GIF, 200, {"Content-Type": "image/gif", "Cache-Control": "no-store"}


app.run(port=8080)